Loading... Please wait...

Categories

Our Newsletter


Repro-nSC (1000x)

  • Repro-nSC
Price:
$129.00
Catalog #:
K6720-500
Quantity:


Product Description

Repro-nSC is a combination of two small molecules: a GSK-3ß inhibitor CHIR99021, and a TGF-ß receptor inhibitor SB431542. A study has shown the efficient conversion of hESCs into homogenous primitive neuroepithelium within 7 days when under synergistic inhibition of GSK-3ß and TGF-ß pathways by Repro-nSC alone or in combination with inhibition of the Notch signaling pathway (e.g. by RO4929097). These primitive neural stem cells can stably self-renew in the presence of LIF and 1x Repro-nSC, retaining high neurogenic potential and responsiveness to instructive neural patterning cues towards midbrain and hindbrain neuronal subtypes, in addition to exhibiting in vivo integration1.


Technical information:

Cocktail Formulation:
1000xDMSO stock solution
  2 mM of SB431542 (TGF-ß1 inhibitor)
3 mM of CHIR99021 (GSK3ß inhibitor)
Purity:   >98% (for each compound)

Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: Store DMSO solution at -20oC.
Usage: Dilute Repro-nSC stock solution (1000x) at 1:1000 into your neural induction media to make 1xRepro-nSC media.


Reference:

1. Li W., et al. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors Proc Natl Acad Sci 2011 108(20):8299-304.

Other Information:

Product Specification (pdf)
MSDS (pdf)

Find Similar Products by Category

Write your own product review

Product Reviews

This product hasn't received any reviews yet. Be the first to review this product!

Add to Wish List

Click the button below to add the Repro-nSC (1000x) to your wish list.

You Recently Viewed...