3-methyladenine (3MA) is a specific inhibitor of autophagy with activity against Vps34 and PI3Kg at IC50 values of 25 and 60 uM, respectively. [1] At concentrations of 5 mM, 3-methyladenine inhibits protein degradation in isolated rat hepatocytes by about 60% without any adverse effects on exogenous protein, protein synthesis, or intracellular ATP levels. [2]
3-methyladenine was shown in time-dependent studies to inhibit class I and class III PI3K in different manners when compared to wortmannin. [3] In an inflammation model setting, 3-methyladenine was found to enhance LPS-induced NF-kB activation and production of TNF-a, iNOS, COX-2, IL-1b, and IL-12. Through the inhibition of Akt by way of class I PI3K interference, 3-methyladenine can positively regulate p38, JNK, and p65, while negatively regulate TBK1 and IFN regulatory factor 3. [4]
Technical information:
| Chemical Formula: | C6H7N5 | |
| CAS #: | 5142-23-4 | |
| Molecular Weight: | 149.15 | |
| Purity: | > 98% | |
| Appearance: | White | |
| Chemical Name: | 3-methyladenine | |
| Solubility: | Up to 22 mM in DMSO | |
| Synonyms: | 3-MA, 3-Methyl-3H-purin-6-amine, 3-Methyl-3H-adenine |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage condition: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Miller et al., Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy, 2010, 6(6), 805-807. Pubmed ID: 20574157 |
| 2. | Seglen et al., 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. 1982, 79(6), 1889-1892. Pubmed ID: 6952238 |
| 3. | Wu et al., Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J. Biol. Chem. 2010, 285(14), 10850-10861. Pubmed ID: 20123989 |
| 4. | Lin et al., Regulation of inflammatory response by 3-methyladenine involves the coordinative actions on Akt and glycogen synthase kinase 3? rather than autophagy. J. Immunol. 2012, 189, 4154-4164. Pubmed ID: 6952238 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
Certificate of Analysis is available upon request.
