This product is the second-generation TOPO cloning kit, which contains the second-generation Topoisomerase, a vector containing the suicide gene ccdB, and a blunt-end factor. Using the second-generation Topoisomerase, with the most suitable buffer, it has higher activity and further improves the cloning efficiency. The efficient connection is completed in 5 minutes at room temperature. Using a vector containing the suicide gene ccdB, when the insert is successfully connected to the vector, the correct expression of ccdB is destroyed, and the host cell can grow normally, otherwise the host cell cannot grow normally, thus achieving a "Zero" background. The addition of blunt-end factor makes this product compatible with TA cloning and blunt-end cloning.
Product Manual (pdf)

Advantages

• Compatible: Compatible with TA cloning and blunt end cloning at the same time;
  Fast: add fragments and react for 5 minutes;
  High efficiency: high cloning efficiency and positive rate>95%.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Xing Y, et al. SLERT Regulates DDX21 Rings Associated with Pol I Transcription. Cell, 2017, 169(4):664-78. Pubmed ID: 28475895
2. Wu N, et al. TBX6 null variants and a common hypomorphic allele in congenital scoliosis. N Engl J Med, 2015, 4793:341-50. Pubmed ID: 25564734
3. Ge J, et al. Architecture of the mammalian mechanosensitive Piezo1 channel. . Pubmed ID: Nature, 2015, 527(7576):64-9. Pubmed ID: 26390154
4. Zhang XO, et al. Complementary Sequence-Mediated Exon Circularization. Cell, 2014, 159(1):134-47. Pubmed ID: 25242744
5. Zong Y, et al. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A. Nature Biotechnology, 2018. Pubmed ID: 30272679
6. Li X, Wang Y, Liu Y, et al. Base editing with a Cpf1-cytidine deaminase fusion. Nature Biotechnology, 2018, 36(4):324-7. Pubmed ID: 29553573

Mechanism

Storage

All components should be Store at -20°C.

Components

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