This kit is mainly intended for genomic DNA extraction from bacteria (Gram-positive or Gram-negative) of a variety of sources. The kit is based on silica gel column purification technology that eliminates the need for extraction using phenol/chloroform organic solvents or time-consuming alcohol precipitation step. With this kit, RNA, proteins, lipids and other inhibitory impurities can be removed at the greatest extent. The DNA obtained can be directly used in PCR, qPCR, enzyme digestion and Southern blot, etc.