This kit is intended for extraction from 1 - 5 ml of overnight bacterial cultures. It adopts an optimized alkaline lysis method and features a unique silica membrane adsorption technology and special Buffer ERB and Buffer ERW for specific binding of plasmids and effective removal of endotoxins, proteins, and other impurities. The whole extraction process takes only 25 min. The extracted plasmid DNA contains minimal endotoxin residue (≤0.1 EU/μg) and can be used for the transfection of various types of cells and common experiments such as enzyme digestion, PCR, sequencing, and ligation.