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FastPure Plasmid Mini Kit - 100 preps | DC201-01

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Product Description

This Kit based on optimized SDS alkaline lysis technology, is applicable for the purification of plasmids DNA from 1 - 5 ml overnight cultures.
By using this kit, the extraction of multiple samples can be finished rapidly within 30 min without phenol chloroform or ethanol precipitation.
The unique Filtration Column contained in the kit bind the plasmid DNA specifically under high-salt and low-pH condition, maximally removing
proteins, genomic DNA, RNA and other impurities. Then the bound plasmid DNA is eluted from silicon membrane in a low-salt and high-pH
buffer (Each column can absorb maximally 35 ug of plasmid DNA). The purified plasmid DNA can be directly used for traditional biological
experiments: enzyme digestion, PCR, sequencing, ligation, transformation, and transfection of sub-culture cells.

Figure 2 shows the supercoiled and restriction enzyme linerized plasmids to demonstrate the premium quality and yield using the FastPure Kit.

Components DC201-01 (100 preps)
RNase A 150 μl
Buffer P1 30 ml
Buffer P2 30 ml
Buffer P3 40 ml
Buffer PW1 60 ml
Buffer PW2 2 × 20 ml
Elution Buffer 20 ml
FastPure DNA Mini Columns 100
Collection Tubes 2 ml 100

Store the RNase A seperately at -20°C, and store all others at room temperature (15-25°C).
*The buffer forms precipitate when stored at low temperature. The precipitates will redissolve after incubate at room temperature or at 37°C for 10 min if necessary, mixed thoroughly before use.

Other Information:
Product Manual (pdf, download)

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