HiScript II Q RT SuperMix for qPCR (Vazyme, #R222) has been specially optimized for qPCR. For example, the ratio of Random primers/Oligo-dT primer is optimized to enable cDNA synthesis at any region of the template RNA and to ensure the repeatability of qPCR results. The cDNA products are compatible for SYBR- or probe-based qPCR, such as ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711) and AceQ U+ Universal Probe Master Mix V2 (Vazyme, #Q513).
The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.
Product Manual (pdf)

Advantages

• SuperMix for two-step RT-PCR/qPCR
• Based on high effective HiScript II Reverse Transcriptase.
• High Stability: the recommended reaction temperature is 42℃-55℃.
• Anchored Oligo(dT)23VN: further improves the specificity, efficiency, and success rate of the first-strand cDNA synthesis.
• Ready-to-use SuperMix.

When order, Cold Shipping option have to be selected, or your order may be delayed.

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Storage

All components should be Store at -20°C. Avoid repeated freezing and thawing.

Components

Related Products

1. HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme #R312)
2. HiScript III RT SuperMix for qPCR (+g DNA wiper) (Vazyme #R323)
3. ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711)
4. AceQ U+ Universal Probe Master Mix V2 (Vazyme, #Q513)