HiScript II Q RT SuperMix for qPCR (Vazyme, #R222) has been specially optimized for qPCR. For example, the ratio of Random primers/Oligo-dT primer is optimized to enable cDNA synthesis at any region of the template RNA and to ensure the repeatability of qPCR results. The cDNA products are compatible for SYBR- or probe-based qPCR, such as ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711) and AceQ U+ Universal Probe Master Mix V2 (Vazyme, #Q513).
The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.
Product Manual (pdf)
When order, Cold Shipping option have to be selected, or your order may be delayed.
All components should be Store at -20°C. Avoid repeated freezing and thawing.