HiScript II Q Select RT SuperMix for qPCR (+gDNA Wiper) (Vazyme, #R233) has been specially optimized for qPCR. The cDNA products are compatible for SYBR- or probe-basded qPCR, such as ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711) and AceQ U+ Universal Probe Master Mix V2 (Vazyme, #Q513).

The Vazyme HiScript II Reverse Transcriptase, optimized from M-MLV (RNase H-) Reverse Transcriptase, is a new generation reverse transcriptase with highly improved heat stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR is specially designed for 2-step RT-qPCR. The 5× Mix contains all necessary components needed for reverse transcription, including Buffer, dNTPs, HiScript II Reverse Transcriptase, RNase inhibitor, and Random primers/Oligo-dT primer mix.