HiScript II Reverse Transcriptase is a new generation reverse transcriptase optimized from the M-MLV (RNase H-) Reverse Transcriptase. Compared with the last generation reverse transcriptase, the thermo-stability of this product is significantly improved. The half-life of HiScript II Reverse Transcriptase at 50℃ is >240 min. At 55℃, it can also be stable for a long time, which significantly benifits the transcription of RNA templates with complex secondary structure. In addition, it has a improved template affinity and cDNA synthesis efficiency. It has a good resistance to most RT-PCR inhibitors and is suitable for long-fragment cDNA amplification (as long as 20 kb).

Product Manual (pdf)

Unit Definition

One unit (U) is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-insoluble material in 10 min at 37℃ with Poly
(rA)-Oligo (dT) as the template/primer.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Wang C, et al. Hsa-miR-1248 suppressed the proliferation, invasion and migration of colorectal cancer cells via inhibiting PSMD10. BMC Cancer, 2022. 22:922. Pubmed ID: 36028821
2. For most updated citations, please view Vazyme product site

Components

Storage

All components should be Store at -20°C.

Components

Related Products

1. 2 × Phanta Max Master Mix (Vazyme #P515)
2. 2X Phanta Max Master Mix (Dye Plus) (Vazyme #P525)
3. ClonExpress Ultra One Step Cloning Kit (Vazyme, #C115)
4. ClonExpress II One Step Cloning Kit (Vazyme, #C112)
5. ClonExpress MultiS One Step Cloning Kit (Vazyme, #C113)
6. ChamQ Universal SYBR qPCR Master Mix (Vazyme, #Q711)