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Hyperactive In-Situ ChIP Library Prep Kit for Illumina (pG-Tn5) | TD901

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Price:
$1,124.00
Catalog #:
TD901
Quantity:


Product Description

Hyperactive In-Situ ChIP Library Prep Kit for Illumina is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology. CUT&Tag technology is a new method for research on protein-genomic interaction by fusion of Protein G or Protein A with engineered ultra-active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG-Tn5 /pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing.
Compared with the traditional protein-genomic interaction research method of ChIP-Seq, CUT&Tag has significant advantages of low cell input, short operation time, high signal-to-noise ratio, good repeatability and is especially suitable for research on early embryo development, stem cells, tumors, and epigenetics.
Product Manual (pdf)


Advantages

  • Easy and Fast    -DNA tagmentation and adapters ligation can be achieved in one step. Sequencing-ready libraries can be generated from live cells within only 9 hours.
  • Less Input Cells Required   -Start from 60 cells or less.
  • No Foreign DNA/RNA Contaimination   -Kits are made with high purity ingredients with no foreign DNA/RNA contaimination.

Selected Product Citations

  1. Wang J, et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell, 2018, 175(7):18871-1901.
  2. Han X, et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell, 2018, 172(5):1091-107.
  3. Zhang L, et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature, 2018, 564(7735):268-72.
  4. Wu J, et al. Chromatin analysis in human early development reveals epigenetic transition during ZGA. Nature, 2018, 557(7704):256-60.
  5. Zheng C, et al. Landscape of Infltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing. Cell, 2017, 169(7):1342-56.
  6. Huang X, et al. Genomic architecture of heterosis for yield traits in rice. Nature, 2016, 537(7622):629-33.
  7. Wu J, et al. The landscape of accessible chromatin in mammalian preimplantation embryos. Nature, 2016, 534(7609):652-7.
  8. Guo X, et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nature Medicine, 2018, 24(7):978-85.
  9. Liu Z, et al. Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. Neuron, 2019, pii: S0896-6273(19)30153-9.
  10. Li X,, et al. Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction. Nature Communications, 2017, 8(1):991.
  11. Yang L, et al. A single-cell transcriptomic analysis reveals precise pathways and regulatory mechanisms underlying hepatoblast differentiation. Hepatology, 2017, 66(5):1387-401.
  12. Wu Y, et al. Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells. Cell reports, 2019, 29(8): 2461-2472. e6.

Mechanism


Storage

All components should be Store at -20°C. Avoid repeated freeze and thaw.


Components


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