Product Description
Hyperactive pA/G-MNase CUT&RUN Assay Kit enables efficient analysis of protein–DNA interactions by PCR or qPCR. Leveraging a hyperactive Protein A/G-fused MNase, it enables antibody-directed targeting and precise DNA cleavage at genomic binding sites, delivering high-resolution results with minimal background. The streamlined workflow ensures robust performance and high success rates across diverse sample types and antibodies, providing a faster and simpler alternative to traditional ChIP-qPCR.
Features
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Broad antibody compatibility: Seamlessly supports antibodies from multiple species and sources.
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Simplified multi-sample workflow: 8-strip tube format enables easy handling and efficient parallel processing.
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Superior performance: Magnetic bead–based purification improves recovery and enhances signal-to-noise ratio.
Performance
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Broad target compatibility
Consistent enrichment across high- and low-abundance targets. Clear differentiation from IgG controls ensures reliable results.
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Cell type compatibility
Consistent CUT&RUN-qPCR performance in 293T, HeLa, and K562 cell lines, with strong enrichment signals clearly distinguished from IgG controls.
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Cell input compatibility
Vazyme #HD103 supports 5,000–500,000 cells for histone modifications and 10,000–500,000 cells for transcription factor targets, enabling flexible application across diverse assay systems.
Components
Storage
BOX 1: Store at 2 ~ 8°C and ship on ice pack.
BOX 2: Store at -30 ~ -15°C. Ship at ≤0°C.
