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Hyperactive pG-Tn5 Transposon for Illumina (4 μM) | S612

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Price:
$1,245.00
Catalog #:
S612-01
Quantity:


Product Description

Hyperactive pG/pA-Tn5 Transposon (S612/S613) is specifically designed for Cleavage Under Targets and Tagmentation (CUT&Tag) technology which is a new method for research on protein-genomic interaction.
Hyperactive pG/pA -Tn5 Transposon is pre-loaded with Adapter, also is the fusion of Protein G with engineered ultra – active Tn5 transposase to form a novel dual-function fusion enzyme (Hyperactive pG/pA-Tn5 Transposase). It precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation, generating fragments used for PCR enrichment or DNA sequencing. Compared with the traditional protein-genomic interaction research method of ChIP-Seq, CUT&Tag has significant advantages of low cell input, short operation time, high signal-to-noise ratio, good repeatability and is especially suitable for research on early embryo development, stem cells, tumors, and epigenetics.

Product Manual (pdf)


Advantages

Product is pre-loaded with Adapter therefore has better stability and does not require dry ice shipping.


Selected Product Citations

  1. Wang J, et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell, 2018, 175(7):18871-1901.
  2. Han X, et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell, 2018, 172(5):1091-107.
  3. Zhang L, et al. Lineage tracking reveals dynamic relationships of T cells in colorectal cancer. Nature, 2018, 564(7735):268-72.
  4. Wu J, et al. Chromatin analysis in human early development reveals epigenetic transition during ZGA. Nature, 2018, 557(7704):256-60.
  5. Zheng C, et al. Landscape of Infltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing. Cell, 2017, 169(7):1342-56.
  6. Huang X, et al. Genomic architecture of heterosis for yield traits in rice. Nature, 2016, 537(7622):629-33.
  7. Wu J, et al. The landscape of accessible chromatin in mammalian preimplantation embryos. Nature, 2016, 534(7609):652-7.
  8. Guo X, et al. Global characterization of T cells in non-small-cell lung cancer by single-cell sequencing. Nature Medicine, 2018, 24(7):978-85.
  9. Liu Z, et al. Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. Neuron, 2019, pii: S0896-6273(19)30153-9.
  10. Li X,, et al. Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction. Nature Communications, 2017, 8(1):991.
  11. Yang L, et al. A single-cell transcriptomic analysis reveals precise pathways and regulatory mechanisms underlying hepatoblast differentiation. Hepatology, 2017, 66(5):1387-401.
  12. Wu Y, et al. Nuclear Exosome Targeting Complex Core Factor Zcchc8 Regulates the Degradation of LINE1 RNA in Early Embryos and Embryonic Stem Cells. Cell reports, 2019, 29(8): 2461-2472. e6.

Components

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Related Products

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  2. Hyperactive pA-Tn5 Transposon (Vazyme #S613)
  3. Hyperactive In-Situ ChIP Library Prep Kit for Illumina (pG-Tn5) (Vazyme, #TD901-01/02)
  4. Hyperactive In-Situ ChIP Library Prep Kit for Illumina (pA-Tn5) (Vazyme, #TD902-01/02)
  5. TruePrep DNA Library Prep Kit V2 for Illumina (50 ng) (Vazyme #TD501)
  6. TruePrep DNA Library Prep Kit V2 for Illumina (5 ng) (Vazyme #TD502)
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