Mut Express® II Fast Mutagenesis Kit V2 is a site-directed mutagenesis system based on ClonExpress® rapid cloning technology. Using this kit, the target plasmid amplification product is digested with DpnI, ClonExpress® recombination and circularized, and then directly transformed to complete the site-directed mutation. The kit consists of Phanta® Max Super-Fidelity DNA Polymerase amplification module and ClonExpress® rapid cloning module. The ultra-high fidelity of Phanta® MaxSuper-Fidelity DNA Polymerase significantly reduces the possibility of introducing new mutations during the amplification process. The ClonExpress® rapid cloning system uses efficient homologous recombination to replace the traditional annealing loop reaction. Highly optimized reaction buffers, fast operating procedures and extremely high success rate make Mut Express® II Fast Mutagenesis Kit V2 the first choice for DNA site-directed mutagenesis.