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Mut Express II Fast Mutagenesis Kit V2 | C214

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Product Description

The Mut Express II Fast Mutagenesis Kit V2 is designed for rapid site-directed mutagenesis based on ClonExpress rapid cloning technology. The PCR amplified target fragments are treated with DpnI to remove the template DNA, and then cyclized and transformed into E. coli to complete the site-directed mutagenesis.

The kit consists of two modules: the Phanta Max Super-Fidelity DNA Polymerase amplification module and the ClonExpress rapid cloning module. The ultra-fidelity of Phanta Max DNA polymerase significantly reduces random mutations during amplification of up to 20 kb in length. The ClonExpress rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. Benefited from the efficient seamless "ligation" of the two PCR products by ClonExpress technology, the kit can generate two separate site mutations in a single mutagenesis reaction. Highly optimized reaction buffer, time-saving operating procedures and high site-directed mutagenesis efficiency make Mut Express II Fast Mutagenesis Kit V2 the preferred kit for DNA site mutagenesis.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Components C214-01 (10 rxn) C214-02 (25 rxn)
2x Phanta Max Buffer 1.25 ml 1.25 ml
dNTP Mix (10 mM each) 20 μl 50 μl
Phanta Max Super-Fidelity DNA Polymerase 20 μl 50 μl
Dpn I (10 U/μl) 20 μl 50 μl
5x ClonExpress II Buffer 40 μl 100 μl
Exnase II 20 μl 50 μl

Store the kit at -20°C or below

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