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Mut Express MultiS Fast Mutagenesis Kit V2

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Price:
$388.00
Catalog #:
C215-02
Quantity:


Product Description

The Mut Express MultiS Fast Mutagenesis Kit V2 is designed for rapid mutagenesis up to 5 seperate sites based on ClonExpress rapid cloning technology. The PCR amplified target fragments are treated with DpnI to remove the template DNA, and then cyclized and transformed into E. coli to complete the site-directed mutagenesis.

The kit is consisted of two modules: the Phanta Max Super-Fidelity DNA Polymerase amplification module and the ClonExpress rapid cloning module. The ultra-fidelity of Phanta Max DNA polymerase significantly reduces random mutations during amplification of up to 20 kb in length. The ClonExpress rapid cloning module allows homologous recombination reactions to replace the conventional annealing ring-forming reactions, requiring much less template and more flexible primer design strategy. Benefited from the efficient seamless "ligation" of the PCR products by ClonExpress technology, the kit can generate up to five separate site mutations in a single mutagenesis reaction. Highly optimized reaction buffer, time-saving operating procedures and high site-directed mutagenesis efficiency make Mut Express MultiS Fast Mutagenesis Kit V2 the preferred kit for DNA multi-site mutagenesis.


Components C215-01 (10 rxn) C215-02 (25 rxn)
2x Phanta Max Buffer 1.25 ml 3 x 1.25 ml
dNTP Mix (10 mM each) 50 μl 125 μl
Phanta Max Super-Fidelity DNA Polymerase 50 μl 125 μl
Dpn I (10 U/μl) 50 μl 125 μl
5x ClonExpress MultiS Buffer 40 μl 100 μl
Exnase MultiS 20 μl 50 μl

Storage:
Store the kit at -20°C or below

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