Necrostatin-1 is a specific ATP-competitive allosteric inhibitor (EC50 = 180 nM) of death domain receptor-associated adaptor kinase (RIP1)1. It selectively blocks a specific programmed cell death pathway, necroptosis, which leads to necrosis. While affecting this distinct cell death pathway, Necrostatin-1 does not present any perturbation of the Fas/TNFR triggered canonical apoptosis cascade. Necrostatin-1 inhibits the necrosis-induced RIP1 and RIP3 interactions in vitro2 and reduces ischemic brain injury in a mouse model of stroke3.
Technical information:
| Chemical Formula: | C13H13N3OS | |
| CAS #: | 4311-88-0 | |
| Molecular Weight: | 259.33 | |
| Purity: | >98% | |
| Appearance: | Light yellow solid | |
| Chemical Name: | 5-((1H-indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one | |
| Solubility: | Up to 50 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Degterev A., et al. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat. Chem. Biol. (2008), 4:313-321 |
| 2. | Sun L., et al. Mixed Lineage Kinase Domain-like Protein Mediates Necrosis Signaling Downstream of RIP3 Kinase. Cell (2012), 148:213-227. |
| 3. | Degterev A., et al. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat. Chem. Biol. (2005), 1:112-119 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
