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- Vazyme Molecular Research
- PCR/qPCR
- Plant Direct PCR Kit | PD105
Product Description
Plant Direct PCR Kit is designed to perform PCR directly from plant leaves, seeds, etc., and can be used for high-throughput screening of
non-polysaccharide and polyphenolic plant samples. The directly engineered DNA polymerase, which maintains high resistance to PCR
inhibitors in plants and extremely high amplification performance, is suitable for amplification of DNA fragments length within 5 kb. The
unique Lysis Buffer A in kit can be used to lyse fresh or frozen plant tissue with easy handling, and the lysate yield can be used as template
with no need of purification. The protective agent contained in the lysate allows the crude product to be efficiently amplified after multiple
freeze-thaw cycles. Amplification can be directly performed as primers and templates are added to pre-prepared 2 × Plant Direct Master
Mix, which reduce pipetting operation and improve the throughput of detection and reproducibility of results. The amplification product is
blunt end and is applicable to the ClonExpress Rapid Cloning Kit (C112/C113/C114).
Product Manual (pdf)
Advantages
When order, Cold Shipping option have to be selected, or your order may be delayed.
Selected Product Citations
Mechanism
Storage
2 × Plant Direct Master Mix should be stored at -20℃ and avoid repeated freezing and thawing; Plant Direct Lysis Buffer can be stored at -20℃ or 2℃ - 8℃.
Components
