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Repro-ACTH2i (1000x)

Price:
$209.00
Catalog #:
K2284
Quantity:


Product Description

ES cell lines can be established nondestructively from single blastomeres, and still retain their pluripotency. The inhibition of endogenous differentiation and activation of proliferation in preimplantation embryos is helpful in establishing ESC lines.

Studies have demonstrated that media containing 2i (PD0325901 and CHIR99021) dramatically increases the efficiency of establishing mESCs1. ACTH 1-24, adrenocorticotropic hormone fragments 1-24 also improved the survival/proliferation of mESCs, and enhanced the derivation of mESCs from single blastomeres2. When used together, ACTH2i synergistically enhanced the derivation of mESC lines from ICR and C57BL/6J 0.5-4.5 days postcoitum denuded intact zygotes, embryos, or isolated blastomeres from 2- to 8-cell embryos when cultured in small volume droplets in KSR ESC medium with feeders. This method is easy to operate and highly efficient3.


Technical information:

Cocktail Formulation:
1000xDMSO stock solution
  10 mM of ACTH 1-24 peptide
3 mM of CHIR99021 (GSK3ß inhibitor), and
0.5 mM of PD0325901 (MEK inhibitor).
Purity:   >98% (for each compound)

Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: Store DMSO solution at -20oC.
Usage: Add/Dilute Repro-ACTH2i (1000x) at 1:1000 into your ESC media to make 1x Repro-ACTH2i media. Make the media fresh before each use.


Reference:

1. Ying QL, et al. The ground state of embryonic stem cell self-renewal. Nature. 2008. 453(7194):519-23.
2. Wakayama S, et al. Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. Stem Cells. 2007. 25(4):986-93.
3. Lee KH, et al. Simple and efficient derivation of mouse embryonic stem cell lines using differentiation inhibitors or proliferation stimulators. Stem Cells Dev. 2012. 21(3):373-83.

Other Information:

Product Specification (pdf)
MSDS (pdf)

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