This combo kit provides all components to complete RNA enrichment (N409 Rip-off rRNA depletion for plant) and VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604 plus VAHTS DNA and RNA clean beads N411 and N412) with a GREAT NEW YEAR PROMOTION 40% DISCOUNT.  This combo kit is specially designed for the preparation of transcriptome libraries for next generation sequencing (NGS) platforms of Illumina®.The kit is universal and suitable for RNA library construction of RNA that have been obtained by Poly(A)-based mRNA enrichment or rRNA depletion . The kit contains two types of cDNA 2nd Strand synthesis buffer, which can be chosen for library construction for non-stranded transcriptome or stranded transcriptome analysis.

This kit combines 2nd Strand cDNA synthesis, end-repair and dA-Tailing into one step, with no need of purification, which greatly simplifies the process of library construction and shortens the operation time.The optimized reaction system improves the library construction efficiency, is compatible with lower-input RNA, and has uniform coverage for different amounts of input-RNA. Libraries of specific sizes, which can be customized, can be obtained after size selections with magnetic beads.

N409 Product Manual (pdf)

NR604 Product Manual (pdf)

N411 Product Manual (pdf)

N412 Product Manual (pdf)

Advantages

• Applicable to multiple species: human, mouse, rat, bacterial (optional), etc.
• Complete rRNA depletion: rRNA depletion rate > 99.9%.
• Adapt to low-input RNA: Starting from 100 ng of total RNA.
• Flexible protocols: Four optimized protocols for fragmentation and size-selection.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Qu W, et al. CPEB3 regulates neuron-specific alternative splicing and involves neurogenesis gene expression.  Aging 2020. Dec 9:12. Pubmed ID: 33318303
2. Sheng N, et al. Cholesterol 25‑hydroxylase protects against experimental colitis in mice by modulating epithelial gut barrier function. Sci Rep 2020 10:14246. PMID: 32859970
3. Ren A, et al. Zbtb20 deficiency causes cardiac contractile dysfunction in mice. FASEB J. 2020 34:13862-13876. PMID: 32844471

Mechanism

Storage

All components should be Store at -20°C.

Components

Related Products

1. VAHTS mRNA Capture Beads (Vazyme #N401)
2. Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme, #N406)
3. Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407)
4. Ribo-off rRNA Depletion Kit (Plant) (Vazyme, #N409)
5. VAHTS RNA Adapters set 1 - set 2 for Illumina® (Vazyme, #N803/N804)
6. VAHTS RNA Multiplex Oligos set 1 for Illumina® (Vazyme, #N323)
7. VAHTS mRNA-seq V3 Library Prep Kit for Illumina® (Vazyme, #NR611)
8. VAHTS Stranded mRNA-seq Library Prep Kit for Illumina® V2 (Vazyme, #NR612)
9. VAHTS DNA Clean Beads (Vazyme, #N411)
10. VAHTS RNA Clean Beads (Vazyme, #N412)