The T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the adjacent 5'-phosphate and 3'-hydroxyl on the blunt or cohesive end of dsDNA. It can also catalyze the linkage of RNA with ssDNA or RNA in double stranded nucleic acids. However, it cannot catalyze linkages between single stranded nucleotides. The T4 DNA Ligase can be used in labelling the 3'-end of RNA, cyclizing RNA and DNA oligonucleotides, cloning of cDNA, and other manipulation of nucleic acids.

Product Manual (pdf)

Components

Unit Definition 

In a ligation reaction system of 20 μl, one unit (U) is defined as the amount of enzyme required to catalyze the ligation of more than 50%
of 6 μg λDNA-HindIII DNA fragments in 30 min at 16℃.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Chen Y, et al. Sequence, structure, and function of the Dps DNA-binding protein from Deinococcus wulumuqiensis R12. Microbial Cell Factories, 2022. 21:132 
2. For most updated citations, please view Vazyme product site

Storage

Store at -30 ~ -15℃ and transport at ≤0℃.

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