The TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme, #TD501/ TD502/ TD503) is specifically designed for next-generation sequencing (NGS) on Illumina® platforms. This kit enables super fast and easy preparation of ready-to-use DNA library for sequencing by adopting a new-type transposase, which convert the complex steps of DNA fragmentation, end repair, dA-tailing, and adapter ligation into a one-step enzymatic reaction, significantly reducing the demanded amount of the initial DNA and shortening the time of library preparation. Three kinds of specifications of this kit are provided for different amount of input DNA: 50 ng (#TD501), 5 ng (#TD502), and 1 ng (#TD503).
Product Manual (pdf)

Advantages

• Super Fast and Easy
• Time-Saving: Library prepared within 90 min.
• Easy-to-Use: one-step enzymatic reaction, no need for physical shearing / sonication.
• High Adaptability to Input DNA
• Applicable for genomic DNA, cDNA, Amplicons from multiple species and PCR products.
• Input DNA: 1 ng - 50 ng.
• High Amplification Uniformity
• Optimized polymerase and buffer to achieve high efficiency and uniformity in library amplification.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Han X, et al. Mapping the Mouse Cell Atlas by Microwell-Seq. Cell, 2018. 172(5):1091-107.. Pubmed ID: 29474909
2. Wu J, et al. Chromatin analysis in human early development reveals epigenetic transition during ZGA. Nature, 2018. 557(7704):256-60.. Pubmed ID: 29720659
3. Zheng C, et al. Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing. Cell, 2017. 169(7):1342-56.. Pubmed ID: 28622514
4. Huang X, et al. Genomic architecture of heterosis for yield traits in rice. Nature, 2016, 537(7622):629-33.. Pubmed ID: 27602511
5. Wu J, et al. The landscape of accessible chromatin in mammalian preimplantation embryos. Nature, 2016, 534(7609):652-7.. Pubmed ID: 27309802
6. Cao S, et al. Chromatin accessibility dynamics during chemical induction of pluripotency. Cell Stem Cell, 2018, 22(4): 529-42.. Pubmed ID: 29625068

Mechanism

Storage

All components should be TD501: store at -20°C; TD502: store 5× TS at room temperature and other components at -20°C; TD503: store 5× TS at room temperature and other components at -20°C. May be shipped at 4°C.

Components

Related Products

1. VAHTS DNA Clean Beads (Vazyme, #N411); (url to N411)
2. TruePrep Index Kit V2 for Illumina® (Vazyme, #TD202)
3. TruePrep Index Kit V3 for Illumina® (Vazyme, #TD203)
4. TruePrep Index Kit V4 for Illumina® (Vazyme, #TD204/TD205/TD206/TD207)
5. VAHTS Universal DNA Library Prep Kit for Illumina® V3 (Vazyme, #ND607)