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VAHTS HiFi Ampification Mix | N616

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Product Description

VAHTS HiFi Amplification Mix (Vazyme, #N407) is a new High-fidelity PCR amplification master mix, which is specially designed for PCR library amplification for next-genenration sequencing (NGS). The master mix is based on VAHTS HiFi DNA Polymerase, which is the new generation of DNA polymerase with high yield and fidelity engineered from Pfu DNA Polymerase. The sensitivity of this enzyme has been greatly improved, with extremely high amplification efficiency and extensive adaptability of the template. It greatly improves the amplification output and the platform period. At the same time, the amplification mismatch rate is 1/52 of Taq DNA Polymerase and 1/6 of Pfu DNA Polymerase, ensuring the fidelity of library amplification. VAHTS HiFi Amplification Mix, with an optimized buffer system, enables low preference of high-throughput sequencing library, wide template input range, high yield, and high stability amplification. This product contains all the components required for PCR amplification, and contains library amplification primers for Illumina sequencing platforms. It can be used easily by only adding a library template. It contains special protective regents, which enables long-term storage and can maintain a stable activity after repeated freezing and thawing. The amplified product is blunt and can be used directly for blunt-end cloning.
Product Manual (pdf)


  • Excellent yield and fidelity in liburary amplification.
  • Applicable to NGS libraries of most input samples: genomic DNA, cfDNA, ctDNA, FFPE DNA, ChIP DNA, Amplicons, Transcriptome.
  • Easy to use: premixed PCR buffer + PCR Primer Mix.
  • Adapt to low-input DNA: starting from 100 pg-5 μg of DNA.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations



All components should be Store at -20°C.


Related Products

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  3. VAHTS mRNA-seq V3 Library Prep Kit for Illumina® (Vazyme, #NR611)
  4. VAHTS Stranded mRNA-seq Library Prep Kit for Illumina® V2 (Vazyme, #NR612)
  5. VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® (Vazyme, #NR603)
  6. VAHTS DNA Clean Beads (Vazyme, #N411)

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