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VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® | NR603

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Product Description

VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina® (Vazyme, #NR603) is specially designed for the preparation of stranded transcriptome libraries for next generation sequencing (NGS) platforms of Illumina. The initial template is prepared with 0.1 µg-1 µg of total RNA of human, mouse, or rat. Compared with traditional transcriptome library preparation kits, this kit enables the depletion of ribosomal RNA (rRNA), including both cytoplasmic (28S, 18S, 5.8S, and 5S) and mitochondrial (16S and 12S) rRNA, leaving mRNA and other non-coding RNA behind. This kit is suitable for subsequent non-coding RNA analysis such as lncRNA. Degraded RNA (i.e. FFPE RNA) could also be used to prepare the library with this kit. In addition, this kit enables the insertion of dUTP during the 2nd strand synthesis of cDNA. The double strand cDNA are digested by uracil-DNA glycosylase (UDG) to remove the second strand before sequencing. As a result, only information from the 1st strand cDNA is preserved. In addition to standard transcriptome information, strand-specific (i.e. from sense or anti-sense DNA) information can also be obtained from NGS data analysis.
Product Manual (pdf)


  • Applicable to multiple species: human, mouse, rat, bacterial (optional), etc.
  • Complete rRNA depletion: rRNA depletion rate > 99.9%.
  • Adapt to low-input RNA: Starting from 100 ng of total RNA.
  • Flexible protocols: Four optimized protocols for fragmentation and size-selection.

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

  1. He YM, et al. Transitory presence of myeloid-derived suppressor cells in neonates is critical for control of inflammation. Nature Medicine, 2018. 24(2):224-23.. Pubmed ID: 29334374



All components should be Store at -20°C.


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