Product Description
VAMNE Magnetic Cell/Tissue Total RNA Kit is used to extract high-purity RNA from cells and animal tissues, achieving high-throughput processing of samples. The kit is based on superparamagnetic particle purification technology and a unique reagent system, which can effectively remove various impurities and specifically adsorb nucleic acids. DNase I can efficiently remove DNA. The automated operation process is simple, fast, and safe. The obtained RNA can be directly used for molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.
Features
Full Automation: Get 96 samples in 40 min. Reagents prefilled in plates.
-
High Compatibility: Get high quality RNA in animal tissues (<30 mg) , cells (1×104 - 5×106).
|
Source |
Amount (mg) |
Yield (μg) |
|
|
Tissue |
Mouse Liver |
9.9 - 10.7 |
65.3 - 66.3 |
|
Mouse Kidney |
10.2 - 10.6 |
41.6 - 43.6 |
|
|
Mouse Spleen |
10 - 10.7 |
96.5 - 104.9 |
|
|
Mouse Lung |
9.8 - 10.4 |
17.6 - 18.3 |
|
|
Mouse Heart |
10.2 |
8.2 - 9.4 |
|
|
Mouse Muscle |
9.6 - 9.8 |
3.1 - 3.2 |
|
|
Mouse Brain |
10.3 - 11.1 |
13.6 - 16.5 |
|
|
Rat Brain |
21.2 - 23.0 |
15.5 - 20.0 |
|
|
Rat Ear |
20.5 - 22.1 |
190.6 - 222.4 |
|
|
Rat Liver |
20.5 - 21.1 |
51.8 - 67.0 |
|
|
Rat Kidney |
21.0 - 22.2 |
20.6 - 23.2 |
|
|
Rat Muscle |
20.2 - 21.6 |
5.4 - 10.1 |
|
|
Rat Lung |
22.0 - 22.9 |
25.2 - 30.6 |
|
|
Rat Bone |
11.2 |
6.4 - 6.6 |
|
|
Rat Fat |
21.2 - 22.5 |
14.4 - 17.0 |
|
|
Female crab gonad |
10.7 - 13.2 |
12.8 - 16.2 |
|
|
Sea cucumber body wall |
20.3 - 20.9 |
5.3 - 5.6 |
|
|
Silver carp skin |
20.2 - 22.0 |
6.6 - 8.6 |
|
|
Silver carp gill |
19.8 - 23.4 |
18.3 - 31.8 |
|
|
Zebrafish |
20.2 - 22.3 |
136.1 - 201.1 |
|
|
Chicken pancreas |
10.0 - 13.4 |
41.9 - 53.8 |
|
|
Gray planthopper |
20.2 - 22.5 |
106.6 - 132.6 |
|
|
Cell |
HeLa |
1.6 × 106 |
48.4 - 54.1 |
|
BV2 |
1.4 × 106 |
36.0 - 39.4 |
|
|
Vero |
2.0 × 106 |
26.2 - 28.9 |
|
|
A549 |
5.6 × 105 |
20.9 - 22.7 |
|
|
HepG2 |
1.0 × 105 |
8.5 - 9.1 |
|
|
HEK293 |
1.9 × 106 |
166.6 - 182.7 |
|
|
SKOV3 |
7.9 × 105 |
9.7 - 9.8 |
|
|
SUM149PT |
1.0 × 105 |
6.9 - 7.0 |
|
|
LNcap |
1.0 × 105 |
7.9 - 8.6 |
|
|
Jurkat |
1.0 × 105 |
4.1 |
|
|
MDCK |
1.4 × 106 |
22.8- 24.2 |
|
|
MCF-7 |
2.9 × 105 |
6.9 - 7.1 |
|
|
SW480 |
1.7 × 106 |
24.5 - 25.5 |
|
|
RPASMC |
4.5 × 105 |
11.3 - 11.8 |
|
|
|
 |
 |
|
Fig.1 RNA preps were performed on different cells using the VAMNE Magnetic Cell/Tissue Total RNA Kit (Vazyme #RMA101 ). The concentration was analyzed by nanodrop. RMA101-purified RNA show excellent yields on different cell lines. The cell input number is 0.5-2 million. |
|
 |
 |
|
Fig.2 Extraction yields from various tissues using different products. |
|
-
Low Toxic: No dangerous goods, such as Trizol or chloroform
Selection Guide
Components
|
Components |
||||||||||
|
BOX 1 |
VAMNE Magnetic Cell/Tissue Total RNA Kit (including Binding Buffer, Beads, Wash Buffer 1, Wash Buffer 2, Wash Buffer 3)
|
|||||||||
|
Lysis Buffer 1 |
||||||||||
|
RDD Buffer |
||||||||||
|
Elution Buffer |
||||||||||
|
BOX 2 |
Proteinase K |
|||||||||
| DNase I |
||||||||||
Storage
Store BOX 1 at 15 ~ 25°C and ship at room temperature.
Store BOX 2 at -30 ~ -15°C and ship at ≤0°C.
Product Manual
