Y-27632 is widely used as a specific inhibitor of the Rho-associated coiled-coil forming protein serine/threonine kinase (ROCK) family. It has been found to prevent dissociation-induced apoptosis in human embryonic stem cells and enhance the survival and cloning efficiency of dissociated hES cells without affecting their self-renewal properties or pluripotency. Y-27632 can also enhance survival during the transplantation of ES cell-derived neural precursors.
Technical information:
| Chemical Formula: | C14H21N3O·2HCl | |
| CAS #: | 146986-50-7 | |
| Molecular Weight: | 329.27 | |
| Purity: | >98% | |
| Appearance: | White solid | |
| Chemical Name: | (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide | |
| Solubility: | Up to 100 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Ishizaki, T. et al. Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol (2000) 57: 976-983. |
| 2. | Koyanagi et al. Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors. J. Neurosci Res (2008) 86: 270-280. |
| 3. | Watanabe et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotech (2007) 25: 681-686. |
Other Information:
Product Specification (pdf)
MSDS (pdf)
