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CCK-8 Cell Counting Kit | A311

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Price:
$99.00
Catalog #:
A311-01
Quantity:


Product Description

CCK-8 Cell Counting Kit, referred to as CCK-8 kit, is dependent on WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5- (2,4-Disulfobenzene)-2H-tetrazole monosodium salt) is a fast, highly sensitive, non-radioactive colorimetric detection kit widely used in cell proliferation and cytotoxicity detection. WST-8 is electronically coupled The carrier 1-Methoxy PMS can be reduced by some dehydrogenases in the mitochondria to form a highly water-soluble orange-yellow formazan product (Formazan). The color of the formazan produced is directly proportional to cell proliferation and inversely proportional to cytotoxicity. .
Use a microplate reader to measure the absorbance at 450 nm wavelength, which can indirectly reflect the number of living cells. The CCK-8 Solution of this kit is a ready-to-use reagent, which can be directly added to the cell sample and can be tested after incubating for a certain period of time. There is no need to pre-prepare various components.


Product Manual (pdf)


Advantages

  • Simple operation; high sensitivity; low cytotoxicity

When order, Cold Shipping option have to be selected, or your order may be delayed.


Selected Product Citations

  1. Guo C, et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity, 2018, 49(5): 842-56.. Pubmed ID: 30366764
  2. Xu L, et al. The transcription factor TCF-1 initiates the differentiation of T(FH) cells during acute viral infection. Nature Immunology., 2015. 16(9): 991-99.. Pubmed ID: 26214740
  3. Liu X, et al. BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3. Gastroenterology, 2017, 153(6):1607-20.. Pubmed ID: 28780076
  4. Qiu W, et al. Deciphering Pancreatic Islet β Cell and α Cell Maturation Pathways and Characteristic Features at the Single-Cell Level. Cell Metabolism, 2017, 25(5):1194-205.. Pubmed ID: 28467935
  5. Zhou H, et al. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nature Neuroscience, 2018. 21(3):440-6.. Pubmed ID: 29335603

Components


Storage

Keep away from light at 2 ~ 8℃. Adjust the shipping method according to the destination.


Components

 


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