ChamQ Universal SYBR® qPCR Master Mix (Vazyme, #Q711), protected by Champagne Taq DNA Polymerase (Vazyme, #P122) via an antibody-modified hot-start activation technique, is specially designed for SYBR Green I based quantitative PCR (qPCR). Unique factors (i.e. the specificity-promoting Exactor) in the optimized buffer system of ChamQ Universal SYBR® qPCR Master Mix significantly improve its sensitivity and specificity. This kit contains unique ROX Passive Reference Dye and is applicable for all Real-time PCR instruments, including instrument that needs no ROX dye, low ROX dye, or high ROX dye. The mix is prepared at 2× reaction concentration and can be directly used for robust and low-template qPCR with high sensitivity, specificity, and reliability.
Product Manual (pdf)

Advantages

• Best combination of specificity + sensitivity.
• Unique antibody-modified hot-start taq, with unique specificity-promoting factors.
• Optimal concentrations of Mg2+ and dye.
• Universal: contains a unique ROX Passive Reference Dye and is applicable for all Real-time PCR instruments (no ROX, low ROX, and high ROX).

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Guo C, et al. Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. Immunity, 2018, 49(5): 842-56.. Pubmed ID: 30366764
2. Xu L, et al. The transcription factor TCF-1 initiates the differentiation of T(FH) cells during acute viral infection. Nature Immunology., 2015. 16(9): 991-99.. Pubmed ID: 26214740
3. Liu X, et al. BMI1 and MEL18 Promote Colitis-Associated Cancer in Mice via REG3B and STAT3. Gastroenterology, 2017, 153(6):1607-20.. Pubmed ID: 28780076
4. Qiu W, et al. Deciphering Pancreatic Islet β Cell and α Cell Maturation Pathways and Characteristic Features at the Single-Cell Level. Cell Metabolism, 2017, 25(5):1194-205.. Pubmed ID: 28467935
5. Zhou H, et al. In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nature Neuroscience, 2018. 21(3):440-6.. Pubmed ID: 29335603

Mechanism

Storage

All components should be Store at -20°C. Protected from light.

Components

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