The product T7 RNA Polymerase (T7 RNA polymerase) is a protein encoded by phage T7 DNA  as expressed in recombinant E. coli, and is a kind of DNA-dependent 5'→3' RNA polymerase with high specificity for recognizing subsequence of T7 promoter. T7 RNA polymerase can utilize single-stranded or double-stranded DNA of the T7 promoter sequence as a template, and NTPs as substrates to synthesize RNA complementary to the single-stranded or double-stranded DNA template  downstream of the promoter. 

This kit employs double-antibody sandwich ELISA to quantify of T7 RNA Polymerase. The elisa plate is coated with monoclonal antibody against T7 RNA Polymerase to form the immobilized antibody. The T7 RNA Polymerase standard and the test sample are added to the  immobilized antibody microplate. After washing, enzyme-labeled reagents labeled with horseradish peroxidase are added to form the "coated antibody - antigen - enzyme labeled detection  antibody" complex. Add the TMB substrate solution after washing to develop color (the TMB substrate solution turns blue under the catalysis of HRP enzyme, and the color finally changes to yellow with an acid. The color intensity is positively correlated with the amount of T7 RNA  Polymerase in the sample).