Nutlin-3a is an antagonist of murine double minute-2 (MDM2). By inhibiting MDM2-p53 interactions (IC50 = 90 nM), Nutlin-3a stabilizes p53 and induces cell cycle arrest and apoptosis in cells with functional p53. [1] Nutlin-3a induces autophagy and upregulation of AMPK in a p53-dependent manner in acute myelogenous leukemia cells (1-5 ?M). [2]
Technical information:
| Chemical Formula: | C30H30Cl2N4O4 | |
| CAS #: | 675576-98-4 | |
| Molecular Weight: | 581.49 | |
| Purity: | > 98% | |
| Appearance: | white | |
| Chemical Name: | 2-Piperazinone, 4-[[(4S,5R)-4,5-bis(4-chlorophenyl)-4,5-dihydro-2-[4-methoxy-2-(1-methylethoxy)phenyl]-1H-imidazol-1-yl]carbonyl]- | |
| Solubility: | Up to 100 mM in DMSO | |
| Synonyms: | Nutlin-3a,(-)-Nutlin-3 |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage condition: For longer shelf life, store solid powder or DMSO solution at -20oC desiccated.
Reference:
| 1. | Vassilev LT, et al. In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science. 2004; 303(5659):844-8 Pubmed ID: 14704432 |
| 2. | Borthakur G, et al. MDM2 Inhibitor, Nutlin 3a, Induces p53 Dependent Autophagy in Acute Leukemia by AMP Kinase Activation.PLoS One. 2015; 10(10):e0139254 Pubmed ID: 26440941 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
Certificate of Analysis is available upon request.