Product Description
T7 CapMax RNA Polymerase, a variant protein encoded by the bacteriophage T7 DNA expressed in the recombinant E. coli, is a DNA-dependent 5'→ 3' RNA polymerase that highly specifically recognizes T7 promoter sequences. This product uses single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) containing T7 promoter sequences as the template and NTPs as the substrate to synthesize RNA complementary to the DNA template strand downstream of the promoter. Compared with wild-type T7 RNA Polymerase, T7 CapMax RNA Polymerase can effectively enhance the capping rate of mRNA products in in vitro co-transcription.
This product is a GMP grade recombinant T7 RNA Polymerase mutant. Throughout the production process, strict control is exercised over process-related impurities such as host proteins, exogenous DNA, RNase, as well as microbial limits and bacterial endotoxins. Ampicillin and any animal-derived raw materials and excipients are not used or added in the entire production process. GMP-compliant production and quality management standards are adopted, ensuring btraceability of the production process and raw materials and excipients, and meeting the requirements for raw materials and excipients in mRNA vaccine production and other related fields.
Features
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Dramatically reduce the cost of capping by increasing the KdCap/KdNTP, either through
- Reducing the Cap analog input by 95%, or
- Utilizing wild type T7 promoter (GG)
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Comparable CQA to T7 WT
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Tested and confirmed across various mRNA sequences
Performance
Components
Storage
Store at -30 ~ -15℃ and ship at ≤0℃.
