BIX-01294 is a selective inhibitor of G9a histone methyl transferase (G9a HMTase) that impairs G9a HMTase and generation of H3K9me2 in vitro. In its inhibition of the histone lysine methyltransferases, BIX-01294 does not compete with cofactor S-adenosyl-methionine. G9a HMTase regulates gene expression including one of the pluripotency genes Oct4.
It is reported that BIX-01294 enhances reprogramming efficiency of neural progenitor cells to the same levels as when four transcription factors (Oct4, Klf4, Sox2 and c-Myc) were introduced to somatic cells for generation of induced pluripotent stem cells.
Technical information:
| Chemical Formula: | C28H38N6O2 | |
| CAS #: | 935693-62-2 | |
| Molecular Weight: | 490.64 | |
| Purity: | >98% | |
| Appearance: | Pale yellow solid | |
| Chemical Name: | 2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl)-6,7-dimethoxy-N-[1-(phenylmethyl)-4- piperidinyl]-4-quinazolinamine trihydrochloride hydrate | |
| Solubility: | Up to 100 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Kubicek, S. et al., Reversal of H3K9me2 by a small-molecule inhibitor for the G9a histone methyltransferase Mol. Cell. 3rd ed., 25, 473-481, (2007). |
| 2. | Shi, Y. et al. A combined chemical and genetic approach for the generation of induced pluripotent stem cells. Cell Stem Cell 2, 525-528, (2008). |
Other Information:
Product Specification (pdf)
MSDS (pdf)
