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HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper) | R312

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Product Description

Hiscript III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper) is an upgraded version of Hiscript II 1st Strand cDNA Synthesis Kit (+ gDNA
wiper) containing a new generation of reverse transcriptase Hiscript III Reverse Transcriptase and Buffer optimized for reverse transcription.
This product improves the efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA
contamination at 42℃ for 2 min, which makes the results more reliable and simplifies qPCR primer design process without the need to
design primers across introns. The kit contains two separated primers: the reverse transcription primers Oligo (dT) 20 VN and Random
hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can synthesize full-length cDNA (up
to 20 kb) for downstream experiments such as cloning, and can also synthesize a highly uniform cDNA for qPCR quantification.

Product Manual (pdf)


When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations


Store at -30℃ to -15℃. Transportation condition is -20℃ to 0℃. 10× RT Mix contains a high concentration of DTT, and there may be precipitation at low temperatures. Restore to room temperature before use and gently shake and mix until the precipitate is redissolved.



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