Hiscript® III RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of Hiscript® II RT SuperMix for qPCR (+gDNA wiper), including
a new generation of reverse transcriptase Hiscript® III Reverse Transcriptase and optimized Buffer. This SuperMix further improves the
efficiency of cDNA synthesis, and is suitable for two-step qRT-PCR detection. The 4 x gDNA wiper Mix in the kit completely removes
residual genomic DNA from the RNA template, ensuring more reliable quantitative results and simplifying qPCR primer design without the
need to design primers across introns; 5 x Hiscript® III QRT SuperMix contains all components required for the reverse transcription
reaction can be rapidly started by adding template RNA and water, and the gDNA wiper is terminated to ensure the integrity of the cDNA.
The product yield is compatible to dye method qPCR and probe qPCR for high performance gene expression analysis.