M-MLV (H-) Reverse Transcriptase is an M-MLV mutant with loss of RNase H activity obtained by site-directed mutation. Compared with wild-type M-MLV, the M-MLV (H-) Reverse Transcriptase exhibits higher thermostability and greater processivity, making it a better choice in long cDNA synthesis and high-temperature RT reaction. M-MLV (H-) Reverse Transcriptase possesses terminal nucleotidyl transferase (TdT) activity and can be used in 5' RACE or other experiments.