Repro-nSC is a combination of two small molecules: a GSK-3ß inhibitor CHIR99021, and a TGF-ß receptor inhibitor SB431542. A study has shown the efficient conversion of hESCs into homogenous primitive neuroepithelium within 7 days when under synergistic inhibition of GSK-3ß and TGF-ß pathways by Repro-nSC alone or in combination with inhibition of the Notch signaling pathway (e.g. by RO4929097). These primitive neural stem cells can stably self-renew in the presence of LIF and 1x Repro-nSC, retaining high neurogenic potential and responsiveness to instructive neural patterning cues towards midbrain and hindbrain neuronal subtypes, in addition to exhibiting in vivo integration1.
Technical information:
| Cocktail Formulation: 1000xDMSO stock solution |
2 mM of SB431542 (TGF-ß1 inhibitor) 3 mM of CHIR99021 (GSK3ß inhibitor) |
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| Purity: | >98% (for each compound) |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: Store DMSO solution at -20oC.
Usage: Dilute Repro-nSC stock solution (1000x) at 1:1000 into your neural induction media to make 1xRepro-nSC media.
Reference:
| 1. | Li W., et al. Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors Proc Natl Acad Sci 2011 108(20):8299-304. |
Other Information:
Product Specification (pdf)
MSDS (pdf)
