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Repro-n3i (1000x) | Neuron differentiation cocktail

  • Repro-n3i
Price:
$199.00
Catalog #:
K6571-500
Quantity:


Product Description

Repro-n3i is a cocktail of three small molecule inhibitors. According to literature1-3, the combination of these inhibitors significantly accelerated the process of deriving postmitotic neurons from human ESCs and iPSCs. When used together with Repro-nLS1, hESCs or hiPSCs rapidly differentiated into neurons with more than 75% efficiency within 10 days of differentiation. The resulting neurons present classical markers and functional properties of human nociceptors, including tetrodotoxin (TTX)-resistant, SCN10A-dependent sodium currents and response to nociceptive stimuli such as ATP and capsaicin1. This differentiation process is rapid, efficient and can be easily scaled-up.


Technical information:

Cocktail Formulation:
1000xDMSO stock solution
  3 mM of CHIR99021 (GSKß inhibitor),
10 mM of DAPT (Notch inhibitor), and
10 mM of SU5402 (FGFR inhibitor)
Purity:   >98% (for each compound)

Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: Store DMSO solution at -20oC.
Usage: Dilute 1000x Repro-n3i stock solution at 1:1000 into your basic ESC/iPSC media to reconstitute 1xRepro-n3i media.


Reference:

1. Chambers SM, et al. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat Biotechnol. 2012. 30(7):715-20.
2. Chambers SM, et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009 27(3):275-80.
3. Yu PB., et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification Nat Med. 2008. 14(12):1363-9.

Other Information:

Product Specification (pdf)
MSDS (pdf)

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