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Catalog #:

Product Description

3-methyladenine (3MA) is a specific inhibitor of autophagy with activity against Vps34 and PI3Kg at IC50 values of 25 and 60 uM, respectively. [1] At concentrations of 5 mM, 3-methyladenine inhibits protein degradation in isolated rat hepatocytes by about 60% without any adverse effects on exogenous protein, protein synthesis, or intracellular ATP levels. [2]

3-methyladenine was shown in time-dependent studies to inhibit class I and class III PI3K in different manners when compared to wortmannin. [3] In an inflammation model setting, 3-methyladenine was found to enhance LPS-induced NF-kB activation and production of TNF-a, iNOS, COX-2, IL-1b, and IL-12. Through the inhibition of Akt by way of class I PI3K interference, 3-methyladenine can positively regulate p38, JNK, and p65, while negatively regulate TBK1 and IFN regulatory factor 3. [4]

Technical information:

Chemical Formula:   C6H7N5
CAS #:   5142-23-4
Molecular Weight:   149.15
Purity:   > 98%
Appearance:   White
Chemical Name:   3-methyladenine
Solubility:   Up to 22 mM in DMSO
Synonyms:   3-MA, 3-Methyl-3H-purin-6-amine, 3-Methyl-3H-adenine

Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage condition: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.


1. Miller et al., Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy, 2010, 6(6), 805-807. Pubmed ID: 20574157
2. Seglen et al., 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. 1982, 79(6), 1889-1892. Pubmed ID: 6952238
3. Wu et al., Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J. Biol. Chem. 2010, 285(14), 10850-10861. Pubmed ID: 20123989
4. Lin et al., Regulation of inflammatory response by 3-methyladenine involves the coordinative actions on Akt and glycogen synthase kinase 3? rather than autophagy. J. Immunol. 2012, 189, 4154-4164. Pubmed ID: 6952238

Other Information:

Product Specification (pdf)
MSDS (pdf)
Certificate of Analysis is available upon request.

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