Loading... Please wait...

Our Newsletter


3-MA

  • 3-MA.jpg
  • 3-MA, 400x400px, png
Price:
$49.00
Catalog #:
C0362-10
Quantity:


Product Description

3-methyladenine (3MA) is a specific inhibitor of autophagy with activity against Vps34 and PI3Kg at IC50 values of 25 and 60 uM, respectively. [1] At concentrations of 5 mM, 3-methyladenine inhibits protein degradation in isolated rat hepatocytes by about 60% without any adverse effects on exogenous protein, protein synthesis, or intracellular ATP levels. [2]

3-methyladenine was shown in time-dependent studies to inhibit class I and class III PI3K in different manners when compared to wortmannin. [3] In an inflammation model setting, 3-methyladenine was found to enhance LPS-induced NF-kB activation and production of TNF-a, iNOS, COX-2, IL-1b, and IL-12. Through the inhibition of Akt by way of class I PI3K interference, 3-methyladenine can positively regulate p38, JNK, and p65, while negatively regulate TBK1 and IFN regulatory factor 3. [4]


Technical information:

Chemical Formula:   C6H7N5
CAS #:   5142-23-4
Molecular Weight:   149.15
Purity:   > 98%
Appearance:   White
Chemical Name:   3-methyladenine
Solubility:   Up to 22 mM in DMSO
Synonyms:   3-MA, 3-Methyl-3H-purin-6-amine, 3-Methyl-3H-adenine

Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage condition: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.


Reference:

1. Miller et al., Finding a fitting shoe for Cinderella: searching for an autophagy inhibitor. Autophagy, 2010, 6(6), 805-807. Pubmed ID: 20574157
2. Seglen et al., 3-Methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc. Natl. Acad. Sci. 1982, 79(6), 1889-1892. Pubmed ID: 6952238
3. Wu et al., Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J. Biol. Chem. 2010, 285(14), 10850-10861. Pubmed ID: 20123989
4. Lin et al., Regulation of inflammatory response by 3-methyladenine involves the coordinative actions on Akt and glycogen synthase kinase 3? rather than autophagy. J. Immunol. 2012, 189, 4154-4164. Pubmed ID: 6952238

Other Information:

Product Specification (pdf)
MSDS (pdf)
Certificate of Analysis is available upon request.

Write your own product review

Product Reviews

This product hasn't received any reviews yet. Be the first to review this product!

Add to Wish List

Click the button below to add the 3-MA to your wish list.

You Recently Viewed...