53AH is a selective Wnt pathway inhibitor. It is a cyclohexyl analog of IWR-1 with defined centers of chirality. In the Wnt assay, the 53AH has 5-fold greater potency in Wnt inhibition. Compared to IWR-1, 53AH is more potent at promoting stem cell differentiation into cardiomyocytes. The increase in Wnt pathway inhibition potency correlates with a 1.4-fold increase in cardiogenesis. 53AH possesses superior physicochemical properties compared to IWR-1 including improved water solubility and significantly increased chemical stability at pH = 7.4 and low cytotoxicity. Accordingly, 53AH is significantly more robust for long-term cell-based experiments and potency in cardiogenesis because of its superior chemical properties.
Technical information:
| Chemical Formula: | C26H28N2O3 | |
| CAS #: | N/A | |
| Molecular Weight: | 416.51 | |
| Purity: | >98% | |
| Appearance: | Off white solid | |
| Chemical Name: | (1R,4r)-4-((2s,3aR,4R,7S,7aS)-1,3-dioxooctahydro-1H-4,7-methanoinden-2-yl)-N-(quinolin-8-yl)cyclohexanecarboxamide | |
| Solubility: | Up to 50 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Willems E, et al, Small-molecule inhibitors of the Wnt pathway potently promote cardiomyocytes from human embryonic stem cell-derived mesoderm. Circ Res. 2011;109(4):360-4. PMID: 21737789 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
