IWP-3 is a selective small molecule wnt inhibitor, prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity1. It blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin. It has an IC50 of 40 nM which is 100 folds less potent than Wnt-C59, but it can still efficiently promote cardiomyocyte differentiation from human ESCs.
Technical information:
| Chemical Formula: | C22H17FN4O2S3 | |
| CAS #: | 687561-60-0 | |
| Molecular Weight: | 484.59 | |
| Purity: | >98% | |
| Appearance: | Light yellow solid | |
| Chemical Name: | 2-(3-(4-fluorophenyl)-3,4,6,7-tetrahydro-4-oxothieno[3,2-d]pyrimidin-2-ylthio)-N-(6-methylbenzo[d]thiazol-2-yl)acetamide | |
| Solubility: | Up to 100 mM in DMSO |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Baozhi Chen, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nature Chemical Biology (2009); 5(2), 100-107. |
| 2. | Willems E, et al. Small-Molecule Inhibitors of the Wnt Pathway Potently Promote Cardiomyocytes From Human Embryonic Stem Cell-Derived Mesoderm. Circ Res. 2011 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
