Repro-n3i is a cocktail of three small molecule inhibitors. According to literature1-3, the combination of these inhibitors significantly accelerated the process of deriving postmitotic neurons from human ESCs and iPSCs. When used together with Repro-nLS1, hESCs or hiPSCs rapidly differentiated into neurons with more than 75% efficiency within 10 days of differentiation. The resulting neurons present classical markers and functional properties of human nociceptors, including tetrodotoxin (TTX)-resistant, SCN10A-dependent sodium currents and response to nociceptive stimuli such as ATP and capsaicin1. This differentiation process is rapid, efficient and can be easily scaled-up.
Technical information:
| Cocktail Formulation: 1000xDMSO stock solution |
3 mM of CHIR99021 (GSKß inhibitor), 10 mM of DAPT (Notch inhibitor), and 10 mM of SU5402 (FGFR inhibitor) |
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| Purity: | >98% (for each compound) |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage: Store DMSO solution at -20oC.
Usage: Dilute 1000x Repro-n3i stock solution at 1:1000 into your basic ESC/iPSC media to reconstitute 1xRepro-n3i media.
Reference:
| 1. | Chambers SM, et al. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat Biotechnol. 2012. 30(7):715-20. |
| 2. | Chambers SM, et al. Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol. 2009 27(3):275-80. |
| 3. | Yu PB., et al. BMP type I receptor inhibition reduces heterotopic [corrected] ossification Nat Med. 2008. 14(12):1363-9. |
Other Information:
Product Specification (pdf)
MSDS (pdf)
