The Ribo-off rRNA depletion kit (Plant) is designed to deplete rRNA (including 5S, 18S and 25S rRNA) from total RNA of plant roots, seeds and leaves and to obtain mRNA and other non-coding RNA. This kit is suitable for removing rRNA from 1 μg - 5μg of plant total RNA. rRNA is depleted by rRNA-to- probe hybridization, RNase H digestion, and DNaseI digestion steps. The retained mRNA and other non-coding RNA can be used for LncRNA and other non-coding RNAs analysis.
This kit contains digestion probes targeting of mitochondrial rRNA and chloroplast rRNA. It can efficiently remove major rRNAs from a variety of plant species and provide the most informative sequencing results. This kit is applicable for both intact and degraded RNA samples. The resulting rRNA-depleted RNA is suitable for RNA library preparation and other downstream experiments.