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T7 Endonuclease I EN303

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Product Description

Product Introduction

T7 Endonuclease I recognizes and cuts mismatched DNA, cruciform DNA, Holliday structures or junctions, and heteroduplex DNA. It can also recognize and cut double-stranded DNA with nicks with a lower speed. The enzyme cuts the first, second or third phosphodiester bond that is 5' to the mismatch. Recombinant T7 Endonuclease I is purified from E.coli expression system. It has high purity and can be applicable for gene mutation, SNP and TALEN or CRISPR/Cas9-mediated mutation detection. It can recognize DNA mismatches, resolve four-way junction or branched DNA, detect heteroduplex or nicked DNA and randomly cleave linear DNA for shot-gun cloning.

Product Advantages

High detection sensitivity;
Products can be directly detected by electrophoresis




Store at -20°C for up to 24 months.

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