Product Description
T7 Pure RNA Polymerase, a variant protein encoded by the bacteriophage T7 DNA expressed in the recombinant E. coli, is a DNA-dependent 5'→3' RNA polymerase that highly specifically recognizes T7 promoter sequences. This product uses single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) containing T7 promoter sequences as the template and NTPs as the substrate to synthesize RNA complementary to the DNA template strand downstream of the promoter. Compared with wild-type T7 RNA Polymerase, T7 Pure RNA Polymerase can significantly reduce the production of dsRNA byproducts during mRNA in vitro transcription.This product is a GMP grade recombinant T7 RNA Polymerase mutant. Throughout the production process, strict control is exercised over process-related impurities such as host proteins, exoge-nous DNA, RNase, as well as microbial limits and bacterial endotoxins. Ampicillin and any animal-derived raw materials and excipients are not used or added in the entire production process. GMP-compliant production and quality management standards are adopted, ensuring traceability of the production process and raw materials and excipients, and meeting the requirements for raw materials and excipients in mRNA vaccine production and other related fields.
Features
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Residual dsRNA < 2 ppm , ~100 × less than T7 WT
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Comparable CQA to T7 WT
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Tested and confirmed across various mRNA sequences
Performance
Components
Storage
Store at -30 ~ -15℃ and ship at ≤0℃.
Product Manual
