VAHTS Small RNA Library Prep Kit for Illumina V2 is a small RNA-seq library preparation kit specifically designed for Illumina high-throughput sequencing platforms. This kit improves the stability and library yield at a lower-input amount through system optimisation. It is intended for animal and plant total RNA with a starting template amount of 10 ng – 1 μg, and ≥1 ng of RNA extracted and purified from serum, plasma, and exosomes. The universal adaptors are ligated to 3′ and 5′ ends of small RNAs, respectively. After steps such as reverse transcription, PCR amplification, and PAGE gel or magnetic bead purification, the sequencing library suitable for the Illumina platform can be obtained. This kit contains all the enzymes and buffers required for library preparation. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.