VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is an RNA-seq library preparation kit specifically designed for Illumina high-throughput sequencing plat_x005f_x0002_forms. The kit is perfectly compatible with total RNA from various types of samples, and the minimum starting input required for this kit is as low as 10 ng.
In addition, this kit combines second-strand synthesis, end repair, and dA- tailing in a single step and eliminates the need for purification during the process, which greatly streamlines the operation workflow and shortens the library preparation time. The kit also provides two types of cDNA Second- Strand Synthesis Buffer for either general or strand-specific RNA-seq library preparation.

Product Manual (pdf)

Components

Applications

VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina is suitable for RNA library construction of total RNA that have been enriched by Poly(A) (for RNA with good integrity from eukaryotes such as animals, plants and fungi) or rRNA depletion. As the content of mRNA in total RNA of different samples varies greatly, enough total RNA need to be inputted to make sure the sufficient mRNA for library construction.  It is recommended to use Agilent 2100 Bioanalyzer to analyze the integrity of total RNA. mRNA enriched with VAHTS mRNA Capture Beads (Vazyme #N401) must be high quality RNA (RIN ≥7). Degraded total RNA used for library construction will lead to 3' bias in RNA-seq. For RNA samples with RIN value <7, rRNA removal can be performed using the Ribo-off method (Vazyme #N406/407/408/409).

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

1. Geng Y, et al. PEAR1 regulates expansion of activated fibroblasts and deposition of extracellular matrix in pulmonary fibrosis. Nature Communications    |    2022 Nov 19    |    PubMed ID: 36402779    | 
2. More citations

Mechanism

Storage

All components should be Store at -20°C.

Related Products

RNA Analysis:
Equalbit RNA HS Assay Kit (Vazyme #EQ211);
Equalbit RNA BR Assay Kit (Vazyme #EQ212);

mRNA Enrichment & rRNA Depletion:
VAHTS mRNA Capture Beads (Vazyme #N401);
Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme #N406);
Ribo-off rRNA Depletion Kit (Bacteria) (Vazyme #N407);
Ribo-off Globin & rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme #N408);
Ribo-off rRNA Depletion Kit (Plant) (Vazyme #N409).

RNA and DNA Clean Beads:
VAHTS DNA Clean Beads (Vazyme #N411) 
VAHTS RNA Clean Beads (Vazyme #N412) 

Adapters:
VAHTS RNA Adapters Set 1 - Set 2 for Illumina (Vazyme #N803/N804),

VAHTS RNA Adapters Set 3 - Set 6 for Illumina (Vazyme #N809/N810/N811/N812)

VAHTS RNA Multiplex Oligos Set 1 - Set 2 for Illumina (Vazyme #N323/N324).

Library Quality Control:
Equalbit 1 × dsDNA HS Assay Kit (Vazyme #EQ121);