VAHTS Universal Plus Fragmentation, End Preparation & dA-Tailing Module for Illumina V2 is a DNA fragmentation, end repair and dAtailing module optimized for library preparation on Illumina high-throughput sequencing platforms. This kit has been optimized and upgraded from the original version. On the basis of maintaining the original high performance, the ratio of Artificial Invert Chimera Reads in the DNA library of FFPE samples has been significantly reduced, and the reliability of SNV detection has been improved. This kit can efficiently fragment DNA templates from 100 pg to 1 μg and perform end repair, phosphorylation of the 5' end, and dA tailing of the 3' end of the fragmented DNA. It significantly reduces the need for starting DNA templates, shortens the experiment time and is compatible with automated library building equipment. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library preparation to the greatest extent.