T4 Polynucleotide Kinase can catalyze the transfer of the γ-phosphate group of ATP to the 5´-hydroxyl end and 3´-monophosphate nucleoside of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA). T4 Polynucleotide Kinase also has 3´-phosphatase activity, which hydrolyzes 3´-phosphate groups from the 3´ phosphate ends of oligonucleotides, deoxy 3´-monophosphate nucleosides and deoxy 3´-diphosphate nucleosides .

Product Manual (pdf)

Components

Unit Definition

One unit (U) is defined as the amount of enzyme catalyzing the incorporation of 1 nmol of [γ-32P] ATP into acid insoluble material in 30 min
at 37℃.

Storage

Store at -30 ~ -15℃.  Transportation at ≤ 0℃. 

When order, Cold Shipping option have to be selected, or your order may be delayed.

Selected Product Citations

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